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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 7, 2014, pp. 1133-1139
Bioline Code: pr14158
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 7, 2014, pp. 1133-1139

 en New Spectrophotometric Methods for the Determination of p-Aminosalicylic Acid in Tablets
Laghari, M.G.H.; Darwis, Y. & Memon, A.H.

Abstract

Purpose: To develop a new spectrophotometric method with improved sensitivity and at higher wavelength for the determination of p-aminosalicylic acid in tablets.
Methods: Two simple and sensitive spectrophotometric methods (methods A and B) were developed using p-dimethylaminobenzaldehyde (DAB), and p-dimethylaminocinnamaldehyde (DAC) as derivatizing reagents for the determination of p-aminosalicylic acid (PAS) in tablets. The derivatization was carried out using 3M HCl-KCl buffer for DAB and 5M HCl-KCl buffer solutions.
Result: The new derivatives of PAS absorbed maximally with bathochromic shift to 460 and 555 nm in the linear concentration range of 0.4 – 2.0 μg/mL with molar absorptivities of 2.4 × 104 and 3.8 × 104 L/mole/cm, respectively, compared to pure PAS which absorbed at λmax of 264 nm with molar absorptivity 7.65 × 103 L/mole/cm in the linear concentration range of 2 - 10 μg/mL. The developed methods were successfully applied to assay PAS in tablets with % recovery of 97.6 ± 1.71 and 98.4 ± 1.45 for methods A and B, respectively.
Conclusion: Both PAS derivatives absorb in the visible spectral region. The presence of excipients in pharmaceutical preparations did not interfere in the determination of PAS as PAS-DAB and PAS-DAC derivatives. Both methods can be applied to determine PAS from bulk and various pharmaceutical dosage forms.

Keywords
p-Aminosalicylic acid; p-dimethylaminobenzaldehyde; p-dimethylaminocinnamaldehyde; Spectrophotometry

 
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