search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 7, 2014, pp. 1149-1155
Bioline Code: pr14160
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 7, 2014, pp. 1149-1155

 en In vitro Antimicrobial and Antibiofilm Activity of Artocarpus Lakoocha check for this species in other resources (Moraceae) Extract against Some Oral Pathogens
Teanpaisan, Rawee; Senapong, Sukunlaya & Puripattanavong, Jindaporn

Abstract

Purpose: To evaluate the antimicrobial and antibiofilm activity of A. lakoocha extract against oral pathogens by an in vitro method.
Methods: The dried powder of the aqueous extract of A. lakoocha was purchased from a Thai traditional drug store. Representative strains of oral pathogens ( Streptococcus mutans check for this species in other resources ATCC 25175, Streptococcus sobrinus check for this species in other resources ATCC 33478, Enterococcus faecalis check for this species in other resources ATCC 19433, Lactobacillus fermentum check for this species in other resources ATCC 14931, Lactobacillus salivarius check for this species in other resources ATCC 11741, Aggregatibacter actinomycetemcomitans check for this species in other resources ATCC 33384, Porphyromonas gingivalis check for this species in other resources ATCC 33277, Prevotella intermedia check for this species in other resources ATCC 25611, Prevotella nigrescens check for this species in other resources ATCC 25261, Fusobacterium nucleatum check for this species in other resources ATCC 25586 and Tanerella forsythia check for this species in other resources ATCC 43037) were tested for minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) using a microdilution technique, as well as by a time kill assay. Antibiofilm activity was investigated by a 3-[4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide (MTT) assay.
Results: All tested strains were susceptible to A. lakoocha extract with variable degrees of antimicrobial inhibition. The extract was effective against both Gram-negative bacteria (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis) and Gram-positive bacteria (Streptococcus mutans, Streptococcus sobrinus), with MIC ranging from 0.10 – 0.39 mg/ml and MBC from 0.10 – 3.12 mg/ml. Killing activity depended on time and concentrations of the extract. The extract acted as a potent antibiofilm agent with dual actions, preventing biofilm formation and also eradicating the existing biofilm.
Conclusion: A. lakoocha extract possesses compounds with good antimicrobial properties that may be used for oral infectious diseases caused by certain oral pathogens associated with dental caries and/or periodontal diseases. For the application, A. lakoocha extract may be incorporated in mouthwash or toothpaste.

Keywords
Artocarpus lakoocha; Antimicrobial; Biofilm; Dental caries; Periodontal diseases; Time-kill assay

 
© Copyright 2014 - Tropical Journal of Pharmaceutical Research
Alternative site location: http://www.tjpr.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2019, Site last up-dated on 12-Jun-2019.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil