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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 8, 2014, pp. 1199-1205
Bioline Code: pr14166
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 8, 2014, pp. 1199-1205

 en Evaluation of Gentamicin-Entrapped Solid Lipid Microparticles Formulated with a Biodegradable Homolipid from Capra hircus check for this species in other resources
Kenechukwu, F.C.; Umeyor, C.E.; Momoh, M.A.; Ogbonna, J.D.N; Chime, S.A.; Nnamani, P.O. & Attama, A.A.

Abstract

Purpose: To formulate solidified reverse micellar solutions (SRMS)-based solid lipid microparticles (SLMs) using homolipid from Capra hircus check for this species in other resources , and evaluate its suitability for the delivery of gentamicin.
Methods: SLMs were formulated by melt-emulsification using SRMS (15 % w/w Phospholipon® 90G in 35 % w/w Capra hircus), PEG 4000 and gentamicin (1.0, 2.0 and 3.0 % w/w), and characterized with respect to size, morphology, encapsulation efficiency (EE) and pH-dependent stability. In vitro release of gentamicin from the SLMs was performed in phosphate buffer (pH 7.4) while bioevaluation was carried out using clinical isolates of Pseudomonas aeruginosa check for this species in other resources and Staphylococcus aureus check for this species in other resources .
Results: Stable and discrete SLMs of size range 1.47 ± 0.02 to 3.55 ± 0.09 ìm were obtained. The SLMs showed a biphasic pattern of drug release and exhibited time-dependent and capacity-limited bioactivity. Overall, SLMs containing 2 % w/w SRMS, 3 % w/w gentamicin and PEG 4000 entrapped the highest amount of drug, released 99 % of drug and gave the highest inhibitory zone diameter (IZD) against the organisms within 420 min, while plain gentamicin gave the least.
Conclusion: SRMS-based SLMs prepared with homolipid from Capra hircus offers a suitable delivery system for gentamicin.

Keywords
Solid lipid microparticles; Gentamicin; Capra hircus; Phospholipon® 90 G; Solidified reverse micellar solution

 
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