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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 8, 2014, pp. 1257-1263
Bioline Code: pr14173
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 8, 2014, pp. 1257-1263

 en Protective Effect of Purple Sweet Potato ( Ipomoea batatas check for this species in other resources Linn, Convolvulaceae) on Neuroinflammatory Responses in Lipopolysaccharide-Stimulated Microglial Cells
Kang, Hyun; Kwak, Yeon-Gil & Koppula, Sushruta

Abstract

Purpose: To evaluate the protective effects of purple sweet potato ( Ipomoea batatas check for this species in other resources Linn, Convolvulaceae) extract (IBE) in stimulated BV-2 microglial cells and its anti-oxidant properties.
Methods: Cell viability assessment was performed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) assay. Lipopolysaccharide (LPS) was used to activate BV-2 microglia. Nitric oxide (NO) levels were measured using Griess assay. Inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 expressional levels were measured by Western blot analysis. Tumor necrosis factor-alpha (TNF-α) production was evaluated by enzyme-linked immunosorbent assay (ELISA). Antioxidant properties were evaluated by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay.
Results: LPS-activated excessive release of NO in BV-2 cells was significantly inhibited by IBE (p<0.001 at 100 μg/mL). Increased production of inflammatory mediators such as iNOS, COX-2 and TNF-α (p < 0.01 and p < 0.001 at 100 and 200 μg/ml, respectively) was attenuated by IBE concentration-dependently. IBE also scavenged DPPH radicals in a dose-dependent manner (p < 0.05 at 10 μg/ml and p < 0.001 at 20 - 200 μg/ml).
Conclusion: These results indicate that IBE attenuated neuroinflammatory responses in LPS-activated BV-2 microglia by inhibiting excessive production of pro-inflammatory mediators such as NO, iNOS, COX-2 and TNF-α. The anti-neuroinflammatory potential of IBE may be related to its strong antioxidant properties.

Keywords
Ipomoea batatas; DPPH radicals; Anti-oxidant; Neuroinflammation; BV-2 microglia; Nitric oxide.

 
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