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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 8, 2014, pp. 1279-1286
Bioline Code: pr14176
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 8, 2014, pp. 1279-1286

 en Comparative Antioxidant, Antiproliferative and Apoptotic Effects of Ilex laurina check for this species in other resources and Ilex paraguariensis check for this species in other resources on Colon Cancer Cells
Pérez, Juan M.; Maldonado, Maria E.; Rojano, Benjamín A.; Alzate, Fernando; Sαez, Jairo & Cardona, Wilson

Abstract

Purpose: To determine and compare the antioxidant, antiproliferative and apoptotic effects of leaf infusions of Ilex laurina check for this species in other resources and Ilex paraguariensis check for this species in other resources in colon cancer cells.
Methods: Antioxidant activity was determined by ORAC (Oxygen Radical Absorbance Capacity) and FRAP (Ferric Reducing Antioxidant Power). Cytotoxic and antiproliferative effects were analyzed using MTT ((3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) and sulfhorodamine-B respectively. Cell death and apoptosis of human colon adenocarcinoma cells SW480 and their metastatic-derived SW620 cells, were analyzed by flow cytometry using propidium iodide and Annexin-V.
Results: Although their flavonoid levels were similar, I. laurina infusion contained 2.2 and 4.4 times higher amounts of total phenolic and caffeoyl derivatives, respectively, than I. paraguariensis. FRAP and ORAC values for I. laurina infusion were 1.6 and 2.0 more active than I. paraguariensis. Both plant infusions inhibited viability and cell growth of SW480 and SW620 cells. These results may be associated to cell cycle-arrest and apoptosis because of the comparable increase of hypodiploid and annexin-V positive colon cancer cells.
Conclusion: These data highlight the antioxidant and promising anticancer activities of I. laurina and Ilex paraguariensis.

Keywords
Ilex laurina; Ilex paraguariensis; Antioxidant; Antiproliferative; Apoptosis; Colon cancer

 
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