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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 9, 2014, pp. 1455-1461
Bioline Code: pr14200
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 9, 2014, pp. 1455-1461

 en In Vitro Screening of Cytotoxic, Antimicrobial and Antioxidant Activities of Clinacanthus nutans check for this species in other resources (Acanthaceae) leaf extracts
Arullappan, Sangeetha; Rajamanickam, Prabu; Thevar, Naadeirmuthu & Kodimani, Clara Carol

Abstract

Purpose: To evaluate the in vitro cytotoxic, antioxidant and antimicrobial activities of Clinacanthus nutans check for this species in other resources extracts and semi-fractions.
Method: The plant was subjected to cold solvent extraction to produce petroleum ether, ethyl acetate and methanol crude extracts, followed by isolation using bioassay-guided fractionation. The crude extracts (0.2 to 10.0 mg/ml) were tested against HeLa and K-562 cell lines using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and antioxidant activity using 1, 1-diphenyl-2- picrylhydrazyl (DPPH) assay. Furthermore, fractions isolated from ethyl acetate leaf extract (0.02 to 10.0 mg/ml) were tested against Bacillus cereus check for this species in other resources (ATCC11778), Escherichia coli check for this species in other resources (ATCC25922), Salmonella enterica check for this species in other resources Typhimurium (ATCC14028) and Candida albicans check for this species in other resources (ATCC10231) using minimum inhibitory concentration (MIC) and minimum bactericidal or fungicidal (MBC/MFC) assays.
Results: Petroleum ether extracts demonstrated the strongest cytotoxic activity against HeLa and K- 562 cells with IC50 of 18.0 and 20.0 μg/mL, respectively. Petroleum ether extracts also displayed the highest radical scavenging activity of 82.00 ± 0.02 %, compared with ascorbic acid and α-tocopherol corresponding values of 88.7 ± 0.0 and 86.6 ± 0.0 %, respectively. In MIC assay, all the crude extracts and fractions showed inhibition against all tested microorganisms. Fraction 7 displayed the lowest MIC and MBC/MFC values against B. cereus and C. albicans at 1.39 mg/ml, respectively. Ampicillin and amphotericin B displayed MIC value of 1.3 mg/ml.
Conclusion: The bioactive compounds from C. nutans are potential cytotoxic, antimicrobial and antioxidant agents.

Keywords
Clinacanthus nutans; Cytotoxic; Antioxidant; Antimicrobial

 
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