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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 9, 2014, pp. 1477-1485
Bioline Code: pr14203
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 9, 2014, pp. 1477-1485

 en Evaluation of the Antioxidant Activities of Ya-hom Intajak, a Thai Herbal Formulation, and its Component Plants
Tuekaew, Jantanarak; Siriwatanametanon, Nisarat; Wongkrajang, Yuvadee; Temsiririrkkul, Rungravi & Jantan, Ibrahim

Abstract

Purpose: To evaluate the antioxidant effect of 80 % ethanol extract and its n-hexane and dichloromethane fractions of Ya-hom Intajak, and its 47 medicinal plants components.
Methods: The 80 % ethanol extract and its n-hexane and dichloromethane fractions were investigated on human low-density lipoprotein (LDL) peroxidation by thiobarbituric acid reactive substances (TBARS) assay. Antioxidant effect was also determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity and ferric reducing antioxidant power (FRAP) assays.
Results: The extract of Ya-hom Intajak exhibited moderate DPPH scavenging activity with an IC50 value of 99.08 μg/ml and a FRAP value of 1.12 mmol FeSO4/g. Among the individual plants, Terminalia chebula check for this species in other resources Retz., Caesalpinia sappan check for this species in other resources L., Cinnamomum bejolghota check for this species in other resources (Buch.-Ham.) and Ci. verum check for this species in other resources J. Presl, showed strong antioxidant activities. T. chebula was the most potent plant in the DPPH assay while Ca. sappan had the highest FRAP value. Among the extracts, the dichloromethane fraction showed the highest inhibitory effect on LDL peroxidation with a half maximal inhibitory concentration (IC50) value comparable to that of probucol (0.82 μg/ml). Pearson correlation analysis revealed that total phenolic content (TPC) showed high positive correlations with FRAP (r = 0.908, p < 0.01) and DPPH activities (r = 0.648, p < 0.01).
Conclusion: Ya-hom Intajak is a valuable source of natural antioxidants and has a potential application as a cardiovascular protective formulation.

Keywords
Ya-hom Intajak; Antioxidant activity; Terminalia chebula Retz.; Caesalpinia sappan L.; Cinnamomum bejolghota (Buch.-Ham.); Ci. verum J. Presl; Phenolic content

 
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