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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 9, 2014, pp. 1487-1493
Bioline Code: pr14204
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 9, 2014, pp. 1487-1493

 en Antioxidant Capacity, Radical Scavenging Kinetics and Phenolic Profile of Methanol Extracts of Wild Plants of Southern Sonora, Mexico
Moran-Palacio, E.F.; Zamora-Álvarez, L.A.; Stephens-Camacho, N.A.; Yáñez- Farías, G.A.; Virgen-Ortiz, A.; Martínez-Cruz, O. & Rosas-Rodríguez, J.A.

Abstract

Purpose: To investigate the antioxidant and phenolic profiles of methanol extracts from Rhizophora mangle check for this species in other resources L, Krameria erecta check for this species in other resources , Lycium berlandieri check for this species in other resources Dunal, Vallesia glabra check for this species in other resources Link and Forchammeria watsonii check for this species in other resources Rose.
Methods: The free radical 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method was used for antioxidant assay of methanol plant extracts. Phenolic compounds determination was carried out by high performance liquid cromatography (HPLC) using an analytical C18 column eluting with acetonitrile on a gradient program.
Results: Extract from Rhizophora mangle L and Krameria erecta displayed 5-fold higher antioxidant capacity than ascorbic acid with DPPH reduction values of 95.71 and 91 %, respectively. Anti-radical kinetic analysis and stoichiometric constant values suggest that the compounds responsible for antioxidant activity are polyphenols and flavonoids with a free or mono-substituted catechol group. Ferulic and vanillic acids had higher contents in Rhizophora mangle L (688.45 ± 18.21 and 7.13 ± 0.125 mg/g, respectively) and Krameria erecta (619.92 ± 28.16 mg/g and 22.29 ± 0.72 mg/g, respectively) than the other constituents.
Conclusion: Rhizophora mangle and Krameria erecta possess five times the antioxidant activity of ascorbic acid and also demonstrate high phenolic contents, which supports the beneficial properties attributed to these plants in traditional medicine, and also justifies the use of the plants as alternative medicines.

Keywords
Rhizophora mangle; Krameria erecta; Ferulic acid; vanillic acid; Antioxidant capacity; Alternative medicines; Phenolics; Anti-radical kinetics

 
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