search
for
 About Bioline  All Journals  Testimonials  Membership  News  Donations


Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 9, 2014, pp. 1495-1501
Bioline Code: pr14205
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 9, 2014, pp. 1495-1501

 en Screening for Anticandidal and Antibiofilm Activity of Some Herbs in Thailand
Kawsud, Pajaree; Puripattanavong, Jindaporn & Teanpaisan, Rawee

Abstract

Purpose: To evaluate the anticandidal activity of the ethanol extracts of 12 herbs from Thailand.
Methods: The herbs studied were Alpinia galanga check for this species in other resources , Curcuma longa check for this species in other resources , Curcuma zedoaria check for this species in other resources , Mentha cordifolia check for this species in other resources , Ocimum africanum, Ocimum basilicum check for this species in other resources , Ocimum sanctum check for this species in other resources , Piper betle check for this species in other resources , Piper chaba check for this species in other resources , Piper nigrum check for this species in other resources , Piper sarmentosum check for this species in other resources and Zingiber officinale check for this species in other resources . Various Candida spp. check for this species in other resources were examined for minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) using microdilution method; time-kill assay was also used to assess the plants. Antibiofilm activity was investigated using a 3-[4, 5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide (MTT assay). Gas chromatography mass spectrometry (GC-MS) analysis, thin layer chromatography (TLC) fingerprinting and TLC-bioautography were used to determine the active anticandidal compounds.
Results: All tested herbs, except extracts of P. nigrum and Limiaceae family, showed varying zones of inhibition against Candida albicans check for this species in other resources ATCC 90028. P. betle revealed the strongest anticandidal activity against all tested strains with MIC ranging from 1.56 to 3.13 mg/ml, and MFC from 3.13 to 8.33 mg/ml. Killing activity depended on time and concentrations of the extract. The concentration of P. betle extract required to inhibit ≥ 90 % biofilm formation of C. albicans ATCC 90028 was 3.13 ± 0.15 mg/ml, while that to remove ≥ 90 % biofilm growth was 12.50 ± 0.69 mg/ml. The result of GC-MS analysis showed the major compound of P. betle extract responsible for anticandidal activity as 4-chromanol.
Conclusion: P. betle extract contains 4-chromanol which is a good potential anticandidal agent for the treatment of oral infectious diseases caused by certain Candida spp.

Keywords
Piper betle; 4-Chromanol; Anticandida; Biofilm; Candidiasis; Herbs

 
© Copyright 2014 - Tropical Journal of Pharmaceutical Research
Alternative site location: http://www.tjpr.org

Home Faq Resources Email Bioline
© Bioline International, 1989 - 2022, Site last up-dated on 19-Jan-2022.
Site created and maintained by the Reference Center on Environmental Information, CRIA, Brazil
System hosted by the Internet Data Center of Rede Nacional de Ensino e Pesquisa, RNP, Brazil