Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 13, No. 9, 2014, pp. 1511-1514
Bioline Code: pr14207
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 13, No. 9, 2014, pp. 1511-1514
© Copyright 2014 - Tropical Journal of Pharmaceutical Research
Spectrophotometric Methods for the Determination of Ketoconazole in Pharmaceutical Dosage Forms|
Fraihat, S.M. & Bahgat, K.M.
Purpose: To develop simple, sensitive and economical methods for the determination of (KZ) in both
pure samples and pharmaceutical formulations.
Methods: The first method (A) was based on the oxidation of the studied drug by a known excess of
cerium (IV) as an oxidizing agent and subsequent determination of unreacted oxidant by reacting it with
indigo carmine (IN) dye. The second method (B) involved potassium permanganate as oxidant and
methylene blue (MB) as dye. Spectrophotometry was used to determine the residual amount of the dyes
by measuring the absorbance of the two dyes at 610 and 660 nm, respectively. Comparison was made
with the British Pharmacopoeia 1998 method.
Results: Linear calibration graphs were obtained at 4 - 30 and 5 - 35 μg/ml for the two methods at 610
and 660 nm, respectively. The regression equation was Y = 0.01X + 0.133, (R2 = 0.996, RSD = 78 %
and limit of detection (LOD) of μg/ml for method A. For the second method (B), it was Y = 0.008X +
0.039, (R2 = 0.995), RSD = 0.95 % and LOD = 2.3 μg/ml. The proposed methods were successfully
applied to the determination of KZ in pharmaceutical formulations.
Conclusion: The results demonstrate that the methods are as accurate and reproducible as official
Ketoconazole; Spectrophotometry; Cerium; Permanganate; Indigo carmine; Methylene blue
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