To assess the anti-hyperlidemic effect of flavone-rich B. chinensis
leaf extract (HTP) in ICR
mice fed a high-fat diet.
HTP (100, 200 and 400 mg/kg) were orally administered to ICR mice fed high-fat diet for 7
weeks. Blood glucose, as well as serum and hepatic levels of lipids were determined at the end of the
experiment. Phosphorylation of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK)
and protein level of peroxisome proliferator-activated receptor α (PPARα) were analyzed by Western
blot and enzyme-linked immunosorbent assay (ELISA) kit, respectively.
Treatment with HTP significantly decreased peri-epididymal fat weight (p < 0.01 and p < 0.05
for 200 and 100 mg/kg, respectively), lowered serum and hepatic lipid, and decreased glucose area
under curve (AUC) in oral glucose tolerance test (p < 0.01 for 200 mg/kg). Western blot and ELISA
analysis showed that administration of HTP (200 mg/kg) significantly increased AMPK (p < 0.05)
phosphorylation and PPARα expression in liver (p < 0.05).
HTP can alleviate hyperlipidemia, at least in part, by up-regulation of AMPK and PPARα.