Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 13, No. 10, 2014, pp. 1667-1673
Bioline Code: pr14229
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 13, No. 10, 2014, pp. 1667-1673
© Copyright 2014 - Tropical Journal of Pharmaceutical Research
Determination of Tolterodine Tartrate in Bulk and Formulation by Extractive Colorimetric Method using Tropaeolin OOO-1|
Ganesh, Mani; Hemalatha, Pushparaj; Peng, Mei Mei; Vinodh, Rajangam; Saktimanigandan, Kalaimani & Jang, Hyun Tae
Purpose: To develop a new simple, accurate, precise and fully validated extractive colorimetric method for the determination of tolterodine tartrate (TL) in bulk and in tablet dosage form.
Method: A chloroform extractable orange red complex formed between the acid dye, tropaeolin OOO-1
and tolterodine in acid media is the basis for this method. The maximum wavelength of absorbance of
the complex was 503 nm. The validation parameters such as stability, accuracy, precision, robustness
and ruggedness were evaluated according to International conference on harmonization (ICH) and
United States Pharmacopoeia (USP) guidelines.
Results: The absorbance of the complex obeyed Beer law over the range 1 - 30 μg/mL with a
correlation coefficient of 0.9945, with a molar absorptivity and Sandal’s sensitivity of 0.0398 and 1.1954
x 104, respectively. The lower limit of detection (LOD) and of quantification (LOQ) of the method were
0.08 and 1 μg mL-1, respectively.
Conclusion: The developed method is validated and has high recovery and precision, and thus is
suitable for routine analysis of the drug in bulk and formulations.
Tolterodine; Tropaeolin; Extractive colorimetry; Validation; Solid dosage
Alternative site location: http://www.tjpr.org