Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 13, No. 11, 2014, pp. 1775-1781
Bioline Code: pr14244
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 13, No. 11, 2014, pp. 1775-1781
© Copyright 2014 - Tropical Journal of Pharmaceutical Research
Improvement of Arbutin Trans-Epidermal Delivery Using Radiofrequency Microporation|
Won, Junho & Park, Jin Woo
Purpose: To assess the ability of radiofrequency (RF) microporation to promote trans-epidermal
delivery of arbutin.
Methods: To investigate the enhancing effect of RF microchannels on skin permeation of arbutin, in vitro skin permeability studies were performed with RF microporation-treated Hartley albino guinea pig
skin using Franz diffusion cell system. Furthermore, improved depigmentation effects in brown guinea
pig in vivo after treatment with RF microporation was evaluated to create hydrophilic microchannels for
arbutin trans-epidermal delivery.
Results: RF microporator caused disruption of the stratum corneum (SC) and created 50 μm diameter
microchannels at a depth of 100 μm in the skin. RF microporation increased skin permeability of arbutin
2.25-fold compared to untreated skin exposed to RF in vitro. Arbutin administration led to considerable
skin depigmentation following RF microporation of UV-induced hyperpigmented skin in a 28-day in vivo
experiment (ΔL-value on day 28: UV, 0.17 ± 0.50; UV + RF, 0.07 ± 0.48; UV + arbutin, 1.32 ± 0.51 and
UV + RF + arbutin, 1.82 ± 0.53).
Conclusion: RF microporation seems to be a suitable physical trans-epidermal delivery technology for
topical application of highly hydrophilic depigmentation agents through the hydrophobic skin barrier.
Radiofrequency microporation; Trans-epidermal delivery; Depigmentation; Arbutin
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