To investigate the anti-hyperlipidaemic effect of extract of Zanthoxylum bungeanum
seed oil (EZSO) on high-fat diet (HFD)-induced hyperlipidemic hamsters.
Following feeding with HFD for 30 days, hyperlipidemic hamsters were intragastrically treated
with EZSO for 60 days. Serum levels of triglyceride (TG), total cholesterol (TC), low-density-lipoproteincholesterol
(LDL-C), nitric oxide (NO) and malondialdehyde (MDA) were analyzed. Protein expression
and location of peroxisome proliferator-activated receptor γ (PPARγ) in liver were determined by
Western blot and immunohistochemical assays, respectively.
EZSO at 5 and 10 g/kg significantly reduced levels of TG by 26 and 23 % (p < 0.05), TC by 19
% (p < 0.01) and 13 % (p < 0.01), LDL-C by 18 % (p < 0.05) and 21 % (p < 0.01), NO by 15 % (p <
0.01) and 31 % (p < 0.01), and MDA by 16 % (p < 0.05) and 30 % (p < 0.01), respectively, in serum of
hyperlipidemic hamsters. However, EZSO did not show significant effect on HDL-C level in serum.
Furthermore, EZSO at 5 and 10 g/kg markedly promoted protein expression of PPARγ by 71 % (p <
0.05) and 102 % (p < 0.01) in liver tissue of hyperlipidemic hamsters. EZSO also notably increased the
content of PPARγ protein in the nucleus of liver cells of hyperlipidemic hamsters.
These findings suggest that EZSO can reduce hyperlipidemia and improve oxidative
stress in hyperlipidemic hamsters through activation of PPARγ, and that EZSO is a promising novel
hypolipidemic health product.