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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 11, 2014, pp. 1911-1915
Bioline Code: pr14263
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 11, 2014, pp. 1911-1915

 en Gas Chromatography-Mass Spectroscopic (GC-MS) Analysis of n-Hexane Extract of Lentinus tuber-regium check for this species in other resources (Fr) Fr (Polyporaceae) Syn Pleurotus tuber-regium check for this species in other resources Fr sclerotia
Afieroho, O.E. & Ugoeze, K.C.

Abstract

Purpose: To identify the chemical constituents of the n-hexane extract of the sclerotia of Lentinus tuber-regium check for this species in other resources (synonym Pleurotus tuber-regium check for this species in other resources ) using gas chromatography-mass spectroscopic (GCMS) techniques.
Methods: The n-hexane extract of the sclerotia of Lentinus tuber regium was obtained by exhaustive Soxhlet extraction and analysed using gas chromatography-mass spectroscopic (MS) techniques. The structures of the identified constituents were confirmed on the basis of their fragmentation pattern in comparison with that obtained from the National Institute of Standards and Technology (NIST) reference library.
Results: Seven fatty acids derivatives: heptadecenal, n-hexadecanoic acid, 1-eicosene, linoleic acid, oleic acid, linoleic acid ethyl ester, and octadecanoic acid, and five steroidal triterpenoids: cholesterol, α- ergostenol, anthraergostatetraenol, stigmasterol, and alpha-ergosta-4,6,8(14),22-tetraen-3-one. The major constituents characterised are α-ergosta-4,6,8(14), 22-tetraen-3-one (8.56 %) > Anthraergostatetra-enol (7.19 %), > n-hexadecanoic acid (6.29 %) > linoleic acid (3.69 %).
Conclusion: This study shows that L tuber-regium is a veritable source of mono- and poly-unsaturated fatty acids, and ergosterol/provitamin D derivatives which may explain, in part, some of its reported nutraceutical benefits.

Keywords
Lentinus tuber-regium; Fatty acids; Steriods; Gas chromatography; Mass spectroscopy

 
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