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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 13, No. 12, 2014, pp. 1993-1997
Bioline Code: pr14274
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 12, 2014, pp. 1993-1997

 en Effect of Triptolide on Functions of Monocytes/ Macrophages in Mildly Acid Microenvironment
Feng, Hongchao; Song, Yufeng; Ma, Hong; Zhang, Yubo & Mao, Benyuan

Abstract


Purpose: To investigate the effects of triptolide (TP) on the proliferation of monocytes/macrophages, secretion of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor-C (VEGFC) in mildly acid microenvironment.
Methods: Human monocytes/macrophage system (THP-1 strains) was cultured under acid (pH 6.6 and pH 6.8) microenvironment and normal microenvironment (pH 7.2) containing different concentrations of TP (5, 10, 20 and 50 μg/mL) for 24, 48 and 72 h. The number of monocytes/macrophages under the varying conditions was subsequently determined by methyl thiazolyl tetrazolium (MTT) assay. The supernatants were collected after 24-h culture, and the content of VEGF and VEGF-C in each supernatant measured by enzyme-linked immunosorbent assay (ELISA).
Results: The proliferation of monocytes/macrophages to some extent enhanced in mildly acid microenvironment. The content of VEGF and VEGF-C declined gradually with increasing TP concentration at the same pH value, and reached a minimum at 50 μg/mL and pH 6.6.
Conclusion: In mildly acid microenvironment, TP promotes the proliferation of monocytes/macrophages to some extent and can reduce their ability to secrete tumor growth promoting factors.

Keywords
Monocytes; Macrophages; Triptolide; Vascular endothelial growth factor; Tumor growth; Tripterygium wilfordii; Sub-acid microenvironment

 
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