Purpose: To investigate the
in-vitro antioxidant and anti-neuroinflammatory effects of
Suaeda maritime
(L) Dumort ethyl acetate (SM-EA) extract in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells.
Methods: LPS-stimulated BV- microglia were used to study the expression and production of
inflammatory mediators,
viz, nitric oxide (NO), inducible NO synthase (iNOS, interleukin (IL)-6) and
tumor necrosis alpha (TNF-α). Antioxidant activity was measured using 1, 1-diphenyl-2-picryl-hydrazyl
(DPPH) assay. Cell viability was estimated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium
bromide (MTT) assay.
Results: SM-EA extract significantly suppressed LPS-induced production of NO (
p < 0.001 at 80 and
100 μg/ml) and expression of iNOS in BV-2 cells. SM-EA also suppressed LPS-induced increase in IL-6
and TNF-α levels (
p < 0.001at 100 μg/ml) in BV- cells. Further, DPPH-generated free radicals were
inhibited by SM-EA extract in a concentration-dependent manner (
p < 0.01 at 0.1 mg/ml and
p < 0.001
at 1 mg/ml) with half maximal inhibitory concentration (IC
50) of 0.42 mg/ml.
Conclusion: The findings imply that SM-EA extract can be developed as a potential therapeutic agent
in regulating microglia-mediated neuroinflammatory responses observed in several neurodegenerative
diseases.
