To improve the production of L-serine from sucrose directly by wild type Corynebacterium glutamicum
The culture medium for the production of L-serine was optimized using a statistical
experimental design. Sucrose, ammonium sulfate ((NH4
) and biotin were the key factors, based on
previous studies. A three-level Box–Behnken factorial design combined with response surface
methodology were used to optimize the medium for the production of L-serine. Validation experiments
were also carried out to establish the adequacy and accuracy of the model obtained.
Under optimal conditions (sucrose 81 g/l, (NH4
30 g/l and biotin 27 μg/l), L-serine
concentration increased to 14.90 g/l which is about 2.5-fold increase over that of the original medium (6
g/l). L-serine concentration in a 5 L fermenter reached 16.4 g/L.
The results show that statistical medium design method is effective in improving L-serine
production. The optimization medium is considered fundamental and useful for the development of C.
SYPS-062 cultivation process for efficient production of L-serine on a large scale.