To evaluate the diuretic activity and acute toxicity profile of the crude aqueous extract of
using animal models.
Albino rats of either sex were divided into five groups (six animals in each group). The control
group received normal saline (10 ml/kg), the reference group received furosemide (10 mg/kg) and the
test groups were administered different doses of the crude aqueous extract (10, 30 and 50 mg/kg) by
intra-peritoneal route, respectively. At the end of 6 h, urine was collected and total volume of urine
excreted by each rat was expressed as ml/6 h/100 g of body weight. pH of fresh urine samples,
concentration of urinary sodium and potassium ions, Lipschitz value, diuretic index, saliuretic index and
ratio were also calculated to make comparison among the groups. The acute toxicity of the
crude extract was assessed in Albino mice.
The findings demonstrated that the crude aqueous extract of the plant showed significant
< 0.001), natriuretic (p
< 0.001) and kaliuretic (p
< 0.001) effects. However, during the course
of the study, urinary pH remained unchanged. The diuretic index values for the test groups (III, IV & V)
were 2.3, 2.6 and 3.1, respectively. Lipschitz values showed that, at the dose of 50 mg/kg, the crude
extract showed 46 % of diuretic activity as compared with furosemide. No toxic effects were observed
among Albino mice even at a higher dose of 3000 mg/kg.
The crude extract of Achyranthes aspera
increases the urine volume and concentration of
urinary electrolytes in a dose-dependent manner. Therefore, this plant has a diuretic potential. However,
future studies should focus on isolating the phytochemical component(s) responsible for diuresis.