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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 13, No. 12, 2014, pp. 2057-2063
Bioline Code: pr14284
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 13, No. 12, 2014, pp. 2057-2063

 en Effect of Extraction Method and Solvent Power on Polyphenol and Flavonoid Levels in Hyphaene Thebaica check for this species in other resources L Mart (Arecaceae) (Doum) Fruit, and its Antioxidant and Antibacterial Activities
Aboshora, Waleed; Lianfu, Zhang; Dahir, Mohammed; Qingran, Meng; Qingrui, Sun; Jing, Li; Al-Haj, Nabil Qaid M. & Ammar, Al-Farga

Abstract


Purpose: To evaluate the influence of extraction method and solvent type on extractable polyphenols and flavonoids in Doum Hyphaene Thebaica check for this species in other resources L Mart (Arecaceae) fruit, as well as to examine the antioxidant and antibacterial activities of the fruit extracts.
Methods: The extraction procedures were performed separately in an ultrasonic bath or shaking water bath for 30 min (70 °C for ethanol and 60 °C for methanol) at agitation speed of 50 Hz and 70 rpm, respectively. The antioxidant potential of the extracts was investigated using β-carotene bleaching, 2,2- diphenylpicrylhydrazyl (DPPH) and reducing power ability assays. In vitro antibacterial activity of the extracts against Staphylococcus aureus check for this species in other resources , Listeria monocytogenes check for this species in other resources , Escherichia coli check for this species in other resources , and Salmonella typhi check for this species in other resources was assessed using agar disc diffusion assay.
Results: Total polyphenol content (TPC) and total flavonoid content (TFC), as well as antioxidant capacity were maximized using methanol as the extraction solvent, particularly with the ultrasonic method. The half maximal inhibitory concentration (IC50) values of the methanol/ultrasonic (MU), methanol/water bath (MW), ethanol/ultrasonic (EU), and ethanol/water bath (EW) extracts in the DPPH assay were 107.6 126.7, 172.7, and 196.3 μg/mL, respectively. The extracts showed strong antibacterial activity against Staphylococcus aureus and Salmonella typhi, while MU extract inhibited the growth of all pathogenic bacteria used in this study.
Conclusion: The antioxidant and antibacterial activities of Doum fruit extracts are significantly affected by the type of extracting solvent and equipment used. The findings further demonstrate that MU extract had stronger antioxidant and antibacterial activity than the other extracts.

Keywords
Doum fruit; Hyphaene thebaica; Ultrasonic extraction; Antioxidant; Phenolic; Flavonoid; Antibacterial

 
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