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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 2, 2015, pp. 241-248
Bioline Code: pr15033
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 2, 2015, pp. 241-248

 en Enhancement of Diosgenin Production in Plantlet and Cell Cultures of Dioscorea zingiberensis check for this species in other resources by Palmarumycin C13 from the Endophytic fungus, Berkleasmium check for this species in other resources sp. Dzf12
Mou, Yan; Zhou, Kaiyi; Xu, Dan; Yu, Ruiting; Li, Jing; Yin, Chunhui & Zhou, Ligang

Abstract

Purpose: To study the effect of palmarumycin C13, an elicitor from the endophytic fungus Berkleasmium sp. Dzf12, on growth and diosgenin production in plantlet or cell cultures of its host plant, Dioscorea zingiberensis check for this species in other resources .
Methods: Palmarumycin C13 was isolated from the ethyl acetate extract of the endophytic fungus Berkleasmium sp. Dzf12 using a combination of high-speed counter-current chromatography (HSCCC), Sephadex LH-20 chromatography and preparative high performance liquid chromatography (HPLC). The biomass of the plantlet and cell cultures of D. zingiberensis as well as their diosgenin content and yield were analyzed after treatment with palmarumycin C13.
Results: Optimal elicitation of diosgenin production by palmarumycin C13 in D. zingiberensis plantlet and cell cultures was achieved when palmarumycin C13 was added to the medium at a concentration of 60 mg/L (for plantlet culture) at the beginning of culturing or 10 mg/L (for cell culture) on day 25 after inoculation. By using these optimal concentrations, the diosgenin yield of the cultured plantlets reached its maximum of 6.44 mg/L, that is, > 1.4-fold increase, while diosgenin yield of the cultured cells reached a maximum of 10.73 mg/L, which is an > 8.0-fold increase.
Conclusion: Addition of palmarumycin C13 from the endophytic fungus, Berkleasmium sp. Dzf12, is a potentially effective strategy for enhancing diosgenin production in D. zingiberensis plantlet and cell cultures.

Keywords
Endophytic fungus; Berkleasmium sp. Dzf12; Spirobisnaphthalene; Palmarumycin C13; Dioscorea zingiberensis; Diosgenin; Elicitation; Cell culture; Plantlet culture

 
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