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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 2, 2015, pp. 279-286
Bioline Code: pr15038
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 2, 2015, pp. 279-286

 en Determination and Distribution Study of Pogostone in Rat Tissues by Ultra - Fast Liquid Chromatography
Chen, Hai - ming; Wan, Lan; Wu, Xiao - li; Li, Chu - wen; Xie, You - liang; Liu, Yu - hong; Liang, Yong - zhuo; Chen, Xiao - ying; Lai, Xiao - ping; Chen, Jian - nan; Li, Yu - cui & Su, Zi - ren

Abstract

Purpose: To develop and validate a rapid, sensitive and reliable ultra - fast liquid chromatography (UFLC) method with photodiode array (PDA) detection for the d etermination of pogostone (PO) in rat tissues using honokiol as internal standard (IS).
Methods: Rats were randomly divided into two groups (intravenous administration group and oral administration group) and given of a single dose of 10 mg/kg PO by intrav enous administration and oral administration, respectively. After intravenous injection, the rats were sacrificed at 15, 60 and 36 0 min, while rats, after oral administration, were euthanasized at 30, 90 and 360 min, respectively. For th e analysis of the p reparation, optimal chromatographic conditions were determined using Acquity UPLC BEH C18 column with acetonitrile - water containing 0.1 % formic acid (55:45, v/v) as the mobile phase, at a flow rate of 400 μL/min. UV detection wavelength was set at 310 nm with temperature maintained at 30 °C.
Results: Good linear relationship of calibration curve (r > 0.9984) was achieved over the range of 0.1 - 40 μg/mL for all the tissue samples. The limit of quantification (LOQ) and limit of detection (LOD) were 0.1 and 0.05 μg/mL, respectively. This method proved to have good precision, accuracy, stability, extraction recovery and matrix effect for tissue d istribution studies of PO in rats.
Conclusion: The developed method is suitable for tissue distribution studies in rats following intravenous and oral administration of PO at a dose of 10 mg/kg

Keywords
Ultra - fast liquid chromatography; issue distribu tion; Pogostone; Honokiol; Rats

 
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