Purpose: To evaluate
in situ macroporous resin adsorption for enhancement of palmarumycins C
12 and
C
13 production in mycelial liquid culture of the endophytic fungus
Berkleasmium
sp. Dzf12.
Methods: Ten macroporous adsorption resins (D-101, D1300, HPD-100, X-5, AB-8, DM130, ADS-17,
DA-201, NKA-9 and S-8) were tested for absorption of palmarumycins C
12 and C
13 in mycelial liquid
culture of the endophytic fungus
Berkleasmium sp. Dzf12.
Results: Among the resins, DA-201 showed the most significant enhancing effect on accumulation of
palmarumycins C
12 and C
13 in mycelial liquid culture of endophytic fungus
Berkleasmium sp. Dzf12.
When resin DA-201 was applied to the medium at a concentration of 4.17 % on day 11 and then
harvested on day 15, the maximal yield of palmarumycins C
12 and C
13 was 149.86 and 55.78 mg/L,
which correspond to 70.69- and 1.82-fold higher than for control (2.12 and 30.70 mg/L, respectively).
Approximately 95.81 % of palmarumycin C
12 and 87.20 % of palmarumycin C
13 were distributed in resin
DA-201.
Conclusion: The results indicate that
in situ resin adsorption is an effective strategy for enhancing the
production of palmarumycins C
12 and C
13, and also for facilitating their recovery from the mycelial liquid
culture of
Berkleasmium sp. Dzf12.
