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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 14, No. 3, 2015, pp. 415-421
Bioline Code: pr15055
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 3, 2015, pp. 415-421

 en In vitro Protective Effect of Ganoderol A Isolated from Ganadermalucidum check for this species in other resources Against Ultraviolet A Radiation and its Anti-inflammatory Properties
Jin, Fujun; Zhao, Hongwei; Yuan, Xiao; Zou, Shiyu; Wang, Qiaoli; Ma, Chungwah; Ren, Zhe & Wang, Yifei

Abstract

Purpose: To evaluate the ultraviolet A (UVA) protection and anti-inflammatory activity of ganoderol A extracted from Ganadermalucidum check for this species in other resources .
Methods: The cytotoxicity and in vitro protective effect of ganoderol A against UVA damage were evaluated by MTT assay. Apoptosis and cell-cycle arrest of NIH/3T3 fibroblast cells were assayed by fluorescence-activated cell sorting (FCS). Expression of monocyte chemotactic protein-1 (MCP-1) and inducible nitric oxide synthase (iNOS) were determined using quantitative real-time polymerase chain reaction (qPCR).
Results: The results indicate that the maximal non-toxic concentration of ganoderol A in NIH/3T3 cells and RAW 264.7 macrophages was 50 and 25 μg/mL respectively. DNA in the tails and tail length decreased by 55 and 70 %, respectively, in the group pretreated with ganoderol A compared with the UVA-treated group. G1 phase cells decreased by 23 %, whereas the number of apoptotic cells returned to normal. The expression of MCP-1 and iNOS declined to 60 and 15 %, respectively, compared with LPS-stimulated group.
Conclusion: Ganoderol A has significant anti-inflammatory activity and protection against UVA damage, thus suggesting that the compound is a candidate for the development of a suitable product to protect skin from UV-induced photoaging.

Keywords
Anti-ultraviolet A; Anti-inflammatory; Ganoderol A; Ganodermalucidum; Photoaging

 
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