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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 3, 2015, pp. 503-509
Bioline Code: pr15066
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 3, 2015, pp. 503-509

 en Antioxidant Activities of Methanol Extract and Solvent Fractions of Marine Macroalga, Avrainvillea erecta (Berkeley) A. Gepp and E.S. Gepp (Dichotomosiphonaceae)
Chai, Tsun-Thai; Kwek, Meng-Tee; Ismail, Nor Ismaliza Mohd; Ooi, Jillian Lean Sim; Amri, Affendi Yang; Manan, Fazilah Abd; Law, Yew-Chye & Wong, Fai- Chu


Purpose: To determine the antioxidant activity of methanol extract (ME) and solvent fractions of Avrainvillea erecta as well as their total phenolic and flavonoid contents.
Methods: The antioxidant activities of ME as well as its chloroform, butanol, and aqueous fractions (CF, BF and WF, respectively) of A. erecta were evaluated via 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and hydrogen peroxide (H2O2) scavenging assays as well as ferric reducing antioxidant power (FRAP) assay. Total phenolic and flavonoid contents were determined spectrophotometrically.
Results: CF and BF possessed equally high DPPH scavenging activity with half-maximal effective concentration (EC50) of 535 and 532 mg/ml, respectively. CF had stronger NO scavenging activity (EC50 743 μg/mL) than ME and BF, although weaker compared with quercetin (EC50 279 μg/ml). CF also produced the highest FRAP value (451 μmol Fe2+/g) among all samples examined. Notably, H2O2 scavenging activity was only found in CF (EC50 387 μg/ml), which was as strong (p > 0.05) as that of gallic acid (EC50 456 μg/mL). BF had the highest total phenolic content while CF had the highest total flavonoid content.
Conclusion: CF of A. erecta, which has the highest flavonoid content of all the extracts evaluated, is a potential source of natural antioxidants, especially hydrogen peroxide scavengers.

Antioxidant; Avrainvillea erecta; Flavonoid; Macroalga; Phenolic

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