Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 14, No. 5, 2015, pp. 769-776
Bioline Code: pr15100
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 14, No. 5, 2015, pp. 769-776
© Copyright 2015 - Tropical Journal of Pharmaceutical Research
Protective Effects of Tetrahydrocurcumin and Curcumin against Doxorubicin and Cadmium-Induced Cytotoxicity in Chang Liver Cells|
Somparn, Nuntiya; Kukongviriyapan, Veerapol; Kukongviriyapan, Upa; Senggunprai, Laddawan & Prawan, Auemduan
Purpose: To investigate the cytoprotective effect of tetrahydrocurcumin, (THC) and curcumin (CUR) on
cytotoxicity induced by doxorubicin and cadmium in Chang liver cells.
Methods: Cytotoxicity was determined by sulforhodamine B assay. The expression of nuclear factorerythroid-
2-related factor 2 (Nrf2) Nrf2 regulated cytoprotecetive enzymes, glutamylcysteine ligase
catalytic subunit (GCLC) and NADP (H): quinone oxidoreductase1 (NQO1) was determined by Western
blot analysis. Nuclear translocation of Nrf-2 was analyzed by immunofluorescence method. The level of
superoxide formation was assayed by chemiluminescence method.
Results: Treatment with THC or CUR significantly induced GCLC and NQO1 expression and the
nuclear translocation of Nrf2. Exposure to doxorubicin (DOX) or Cd for 24 h induced cell death of about
50 %. However, pre-treatment with THC or CUR (1 or 6 μM) for 24 h significantly increased cell survival
to 80 or 90 %, respectively (p < 0.05). Similar pre-treatment with THC or CUR significantly protected
against Cd-induced cell death by a level of 80 and 85 %, respectively (p < 0.05). The cytoprotective
effect of these compounds was associated with suppressed DOX- and Cd-induced superoxide
formation and induction of GCLC and NQO1 expression.
Conclusions: THC mediates its effects by activation of Nrf2 and its regulated enzymes, GCLC and
NQO1. Induction of GCLC, NQO1 protein expression and suppression of superoxide are associated
with the cytoprotective effect.
Chang hepatocyte; Curcumin; Tetrahydrocurcumin; Cytoprotection; Doxorubicin; Cadmium
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