To evaluate the anti-tumor activity and explore the possible mechanisms of action of
paederosidic acid isolated from Paederia scandens
Paederosidic acid (PA) was isolated from P. scandens and identified by spectroscopic
methods. The cytotoxic effects of PA in gastric cancer cell lines (MGC-803, BGC-823, and SGC-7901
cells) were assayed using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT).
Apoptosis of PA in SGC-7901 cells was evaluated by DAPI staining. To investigate the possible
mechanisms of apoptosis, the effect of PA on C-caspase-3, C-caspase-9, Bcl-2 and Bax expressions in
SGC-7901 cells were assayed by Western blot analysis.
PA exerted significant inhibitory effects on MGC-803, BGC-823, and SGC-7901 cells with 50
% inhibitory concentration (IC50) values of 42.2, 43.7, and 30.5 μM, respectively, and in a
concentration-dependent manner. Subsequently, SGC-7901 cells were selected for further studies.
After treatment with PA, obvious condensation of the nucleus was observed in fluorescence
photomicrographs, which is a characteristic of apoptosis. In addition, caspase-3, caspase-9 proteins
and Bax were significantly up-regulated (p < 0.05), whereas Bcl-2 was significantly down-regulated (p <
0.05) by PA in a concentration-dependent manner.
PA has significant anti-tumor activity on SGC-7901 cells in vitro, and the possible
mechanism of action may be related to PA-induced apoptosis via mitochondria-mediated apoptosis