Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 14, No. 6, 2015, pp. 997-1003
Bioline Code: pr15130
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 14, No. 6, 2015, pp. 997-1003
© Copyright 2015 - Tropical Journal of Pharmaceutical Research
Identification of Putative Vero Cell Protein(s) that Bind Specifically to Recombinant Envelope Protein of Dengue Virus Type 2|
Zargar, Seema; Wani, Tanveer A & J ain, SK
To identify protein targets in host (vero) cell since there is currently no the
rapy or a licensed
tetravalent vaccine to combat all the four virus serotypes of dengue virus.
The domain III of the dengue virus encoded envelope protein was expressed in pET28a
expression vector and the purified recombinant protein was labeled
with biotin without altering its
immunogenicity. Vero cell proteins on nitrocellulose membrane reacted with recombinant envelope
protein domain III to identify viral target proteins in vero cells.
The 45 KDa, 43 KDa and 30 KDa plasma membrane proteins were identified as viral envelope
targets. Competitive binding assay showed these proteins competing with dengue virus binding. MTT
assay indicate that viability of vero cells increases in cultures pretreated with 45 KDa, 43 KDa and
KDa proteins before dengue infection.
These results indicate the possible role of these proteins in viral binding to vero cells. The
study provides a preliminary insight that would aid in determining the target epitopes against prote
domain III of dengue virus and hence, formulation of a vaccine for preparing neutralizing antibodies.
Dengue virus envelope; Biotinylation; Ni - NTA purification; Target epitopes; Plaque assay; Competitive blocking assay
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