Ameliorative Effects of Neurolytic Celiac Plexus Block on Stress and Inflammation in Rats with Partial Hepatectomy|
Li, Jun; Li, Fu - Xiang; Che, Jian - Xiang; Wei, Xiao - Hong; Qiu, Qing - Ming; Zha, Peng; Bai, Shu - Rong; Tang, Xu - Dong & Chen, Tao
To investigate effects of neurolytic celiac plexus block (NCPB) on stress and inflammation in
rats with partial hepatectomy (PH).
A model of PH rat was established, and serum C-reactive protein (CRP); corticosterone
(GC); adrenocorticotropin (ACTH); noradrenaline (NA); adrenalin (AD); aspartate transaminase (AST);
alanine transaminase (ALT); as well as tumor necrosis factor-α (TNF-α); interleukin (IL)-1β and IL-6; high mobility group box1 (HMGB1); and nitric oxide (NO) concentrations in serum assessed after PH.
Additionally, Western blotting was performed to determine the effect of NCPB on express
glucocorticoid receptors (GR), inhibitor of nuclear factor kappa B (IκB), p65, c-Jun and inducible nitric
oxide synthase (iNOS) of PH rats, as well as assay effects of NCPB on nuclear translocation of GR, c-Jun and p6.DNA binding activities of nuclear factor kappa B (NF-κB) and activator protein 1 (AP-1)
were also determined.
NCPB reduced AST and ALT (P < 0.05), decreased secretion of inflammatory cytokines and
NO (P < 0.05), as well as decreased CRP, GC, ACTH, NA and AD after PH (p < 0.05). NCPB increased expressions of GR and IκB, but expressions of p65, c-Jun, and iNOS (p < 0.05). Additionally, NCPB
increased nuclear translocation of GR (p < 0.01), but decreased nuclear translocation of p65 and c-Jun
after PH (p < 0.05). Additionally,
DNA binding activity of NF
κB and AP
1 was decreased by NCPB (p <
The results indicate that NCPB treatment can significantly inhibit str
ess and inflammation
in PH rats.
Neurolytic celiac plexus block; Cytokine; Nuclear translocation; Partial hepatectomy; Stress; Inflammation