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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-5996
Vol. 14, No. 6, 2015, pp. 1041-1047
Bioline Code: pr15136
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 6, 2015, pp. 1041-1047

 en Carum carvi check for this species in other resources Linn (Umbelliferae) Attenuates Lipopolysaccharide - Induced Neuroinflammatory Respons es via Regulation of NF-κB Signaling in BV-2 Microglia
Kopalli, Spandana Rajendra & Koppula, Sushruta

Abstract

Purpose: To investigate the anti - neuroinflammatory properties of Carum carvi check for this species in other resources Linn. (CCE, Umbelliferae) aqueous extract in stimulate d BV - 2 microglial cells and explore its underlying mechanisms.
Methods: Cell viability assessment was performed by 3 - (4,5 - dimethylthiazol - 2 - yl) - 2,5 - diphenyl - tetrazolium bromide (MTT) assay. Lipopolysaccharide (LPS) was used to activate BV - 2 microglia. Nitric oxide (NO) levels were measured using Griess assay. Inducible NO synthase (iNOS) and cyclooxygenase (COX) levels were evaluated by Western blot analysis. Interleukin - 6 (IL - 6) and tumor necrosis factor - alpha (TNF - α) production were evaluated by enzym e - linked immunosorbent assay (ELISA).
Results: CCE alone did not exhibit any signs of cytotoxicity to BV - 2 cells up to 200 μg/ml concentration. The LPS - activated excessive release of NO in BV - 2 cells was significantly inhibited by CCE (p < 0.001 at 100 μg /mL). CCE also inhibited the production of inflammatory mediators such as iNOS, COX - 2, IL - 6 and TNF - α (p < 0.05, p < 0.01 and p < 0.001 at 25, 50 and 100 μg/mL, respectively). Further mechanistic study revealed that CCE acts by regulation of nuclear factor kappa - B (NF - κB) signaling pathway in LPS - stimulated BV - 2 microglial cells.
Conclusion: The results reveal that CCE exhibited its anti - neuroinflammatory effects via regulation of NF - κB signaling. This can be developed as a potential therapeutic target in ameliorating microglia - mediated neuroinflammation.

Keywords
Carum carvi; Anti - oxidant; Neuroinflammation; Microglia; Nitric oxide; Interleukin

 
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