Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 14, No. 6, 2015, pp. 1095-1101
Bioline Code: pr15144
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 14, No. 6, 2015, pp. 1095-1101
© Copyright 2015 - Tropical Journal of Pharmaceutical Research
Development of a Visible Spectrophotometric Method for the Analysis of Ganciclovir in Bulk Sample and Dosage Form|
Thomas, Olusegun E. & Adegoke, Olajire A.
To develop and validate a simple visible spectrophotometric method for the quantitative
determination of ganciclovir in bulk sample and dosage form.
The method was based on the diazo coupling reaction between diazotized ganciclovir
acidified p-dimethylaminobenzaldehyde. Various analytical parameters for the azo adduct were
established. Validation of the new method was carried out using current ICH guidelines with parameters
including linearity, repeatability, reproducibility and
selectivity determined. The developed method was
thereafter applied to determine ganciclovir in a commonly available brand.
Coupling reaction generated a yellow
coloured product in an alcohol medium with optimal
wavelength at 404 nm. Linear co
rrelation was obtained at concentrations of 10.3
25.7 μg/mL. The
method was accurate and precise with recovery in the range of 99.37
103.15 % while intra
day precision (% RSD) at three different concentrations was < 2.7 %. The limits of det
quantification were 0.23 and 0.70 μg/mL, respectively. When applied to the analysis of the dosage form,
there was no statistically significant difference between the new method and the official HPLC method.
The method is simple, in
expensive, reproducible and fast, and can be employed as a
reliable alternative to the official method for the routine analysis of ganciclovir in bulk and dosage forms.
Ganciclovi; p-dimethylaminobenzaldehyde; Diazo coupling reaction; Absorpt ion spectrophotometry
Alternative site location: http://www.tjpr.org