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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 7, 2015, pp. 1167-1173
Bioline Code: pr15153
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 7, 2015, pp. 1167-1173

 en Molecular Detection of Strongyloides ratti in Faecal Samples from Wild Rats in Serdang, Malaysia
Al-Zihiry, Khalid Jameel Kadhim; Aliyu, Mahmuda; Atshan, Salman Sahab; Unyah, Zasmy; Ibraheem, Zaid Osama; Majid, Roslaini Abdul; Hamat, Rukman Awang & Abdullah, Wan Omar

Abstract

Purpose: To detect Strongyloides ratti in faecal samples using conventional methods and to confirm the identification using a sensitive and specific method, namely, polymerase chain reaction (PCR).
Methods: A PCR method targeting the small subunit of the rRNA gene was performed in this study for the detection of DNA from Strongyloides ratti (an animal model of S. stercoralis check for this species in other resources ) in faecal samples of wild Brown rats, Rattus norvegicus check for this species in other resources .
Results: Strongyloides ratti was detected in 34.2 % of collected rats by different conventional techniques and confirmed by PCR. The essay presented 100 % sensitivity with Strongyloides check for this species in other resources universal primer.
Conclusion: The findings of this study suggest that the application of PCR with universal primer is a very sensitive methodology to detect S. ratti in faecal material of wild rats infected even with very low parasite burden.

Keywords
Faecal; Parasite; Strongyloides stercoralis; Strongyloides ratti; Brown rat; DNA; Universal primer

 
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