Purpose: To optimize the extraction technology of polysaccharides from
Cynomorium songaricum
Rupr
by ultrasonic-assisted extraction (UAE).
Methods: Four parameters including ultrasonic power, ratio of raw material to water, extraction
temperature, and extraction time were optimized by orthogonal design. The effects of the factors on the
yield of polysaccharides were also studied. The hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH)
radical scavenging activities were determined
in vitro by spectrophotometry.
Results: The optimal conditions were as follows: 1:30 as ratio of raw material to water, extraction for 70
min at 80 ℃ with ultrasonic power being 420 W. Under these conditions, the yield of polysaccharides
was up to 4.51 %, which was significantly higher than that obtained under the initial conditions (3.82 %).
DPPH radical scavenging activity reached 64.82 % at 0.012 mg/mL, while hydroxyl radical scavenging
activity was 18.36 % at 0.5 mg/mL.
Conclusion: Ultrasonic-assisted extraction technology is a useful tool for the extraction of bioactive
components from biological materials.
