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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 7, 2015, pp. 1175-1181
Bioline Code: pr15154
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 7, 2015, pp. 1175-1181

 en Application of Orthogonal Design to Optimize Extraction of Polysaccharide from Cynomorium songaricum check for this species in other resources Rupr (Cynomoriaceae)
Wang, Xiaoli; Wang, Fan; Jing, Yanjun; Wang, Yonggang; Lin, Peng & Yang, Lin

Abstract

Purpose: To optimize the extraction technology of polysaccharides from Cynomorium songaricum check for this species in other resources Rupr by ultrasonic-assisted extraction (UAE).
Methods: Four parameters including ultrasonic power, ratio of raw material to water, extraction temperature, and extraction time were optimized by orthogonal design. The effects of the factors on the yield of polysaccharides were also studied. The hydroxyl and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were determined in vitro by spectrophotometry.
Results: The optimal conditions were as follows: 1:30 as ratio of raw material to water, extraction for 70 min at 80 ℃ with ultrasonic power being 420 W. Under these conditions, the yield of polysaccharides was up to 4.51 %, which was significantly higher than that obtained under the initial conditions (3.82 %). DPPH radical scavenging activity reached 64.82 % at 0.012 mg/mL, while hydroxyl radical scavenging activity was 18.36 % at 0.5 mg/mL.
Conclusion: Ultrasonic-assisted extraction technology is a useful tool for the extraction of bioactive components from biological materials.

Keywords
Cynomorium songaricum; Polysaccharide; Ultrasonic-assisted extraction; Orthogonal design

 
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