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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 7, 2015, pp. 1199-1205
Bioline Code: pr15157
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 7, 2015, pp. 1199-1205

 en Cytotoxic Effect and Antioxidant Activity of Bioassay-guided Fractions from Solanum nigrum check for this species in other resources Extracts
Arullappan, Sangeetha; Rajamanickam, Prabu; Thevar, Naadeirmuthu; Narayanasamy, Dhanendiren; Yee, Hiah Yee; Kaur, Parveen & Mahandan, Meerasri


Purpose: To evaluate the cytotoxic effect and antioxidant activity of bioassay-guided fractions from Malaysian species of Solanum nigrum check for this species in other resources .
Methods: Methanol leaf and ethanol fruit extracts of Solanum nigrum were subjected to bioassayguided fractionation using column chromatography. The semi-purified fractions were investigated for their in vitro cytotoxic effect against various cancer cell lines using 3-(4, 5-dimethyl-2-thiazolyl)-2, 5- diphenyl-2H-tetrazolium bromide (MTT) assay, and for antioxidant activity using 2, 2-diphenyl-1- picryhydrazyl (DPPH) assay.
Results: From bioassay-guided fractionation, 13 and 17 fractions were obtained from the methanol leaf and ethanol fruit extracts, respectively. In MTT assay, fractions 1, 2 and 4 from methanol extract showed the highest cytotoxic effect against the cancer cells with IC50 of 13.0 μg/mL at 48 h incubation. For the ethanol extract, fractions 14 and 15 showed the highest cytotoxic effect with IC50 of 12.0 μg/mL against K-562 cells, while fractions 13, 14 and 17 showed IC50 of 13.0 μg/mL against HeLa cells. Doxorubicin hydrochloride and vinblastine sulfate inhibited the cancer cells with IC50 range of 1.3 to 17.0 μg/mL. The highest radical scavenging activity was exhibited by fraction 2 from methanol extract with ED50 value of 0.10 mg/ml, while fraction 15 from ethanol extract showed ED50 of 0.79 mg/mL. Ascorbic acid and α-tocopherol exhibited radical scavenging activity of 95.0 ± 0.01 % (ED50 = 0.05 mg/mL) and 93.0 ± 0.01 % (ED50 = 0.10 mg/mL), respectively.
Conclusion: Solanum nigrum leaves and fruits are potential sources of cytotoxic and antioxidant agents.

Solanum nigrum; Cytotoxic activity; Anti-oxidant activity; Bioassay-guided fractionation; Doxorubicin; Vinblastine

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