Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 14, No. 7, 2015, pp. 1257-1264
Bioline Code: pr15165
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 14, No. 7, 2015, pp. 1257-1264
© Copyright 2015 - Tropical Journal of Pharmaceutical Research
Isolation and Identification of an Antimutagenic Phthalate Derivative Compound from Octopus (Paraoctopus limaculatus)|
Cruz-Ramírez, Susana-Gabriela; López-Saiz, Carmen-María; Plascencia-Jatomea, Maribel; Machi-Lara, Lorena; Rocha-Alonzo, Fernando; Márquez-Ríos, Enrique & Burgos-Hernández, Armando
Purpose: To isolate and evaluate the antimutagenic properties of compounds previously identified in
octopus (Paraoctopus limaculatus).
Methods: Octopus fractions, previously obtained by a sequential thin layer chromatography (TLC)
procedure, were subjected to further fractionation by TLC and their anti-mutagenic activity monitored
using Salmonella tester strains TA98 and TA100 with metabolic activation (S9) in Ames test. The
isolated fractions were subjected to structural studies by Fourier transformed infrared spectroscopy (FTIR),
nuclear magnetic resonance (1H and 13C NMR), and gas chromatography-mass spectrometry.
Results: Five new fractions were obtained from a previously isolated and reported anti-mutagenic
octopus fraction. Fractions RB21321b2 and RB21321b3 inhibited > 80 % of the mutagenicity induced by
500 ng AFB1 on both tester strains and were selected for chemical/structural characterization. Data
from IR and 1H and 13C NMR suggested the presence of phthalate type of compounds. GC-MS analysis
revealed 278 m/z for both fractions which is consistent with a butyl isobutyl phthalate structure.
Conclusion: Based on the findings, the compound responsible for the high anti-mutagenic activity of
the isolated fraction from octopus is 1-butyl-2-isobutyl-phthalate.
Octopus; Anti-mutagenic; Paraoctopus limaculatus; 1-Butyl-2-isobutyl-phthalate
Alternative site location: http://www.tjpr.org