Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 14, No. 8, 2015, pp. 1323-1332
Bioline Code: pr15174
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 14, No. 8, 2015, pp. 1323-1332
© Copyright 2015 - Tropical Journal of Pharmaceutical Research
Preparation, Characterization and In vitro Evaluation of Theophylline Nanoparticles Prepared with Dextran- Conjugated Soy Protein|
Jin, Bei; Zhou, Xiaosong; Chen, Caiyan; Zhang, Xiaosa & Chen, Siqiao
To design and characterize theophylline nanoparticles synthesized with dextran-conjugated
soy protein isolate (SPI), and evaluate their encapsulation capacity and release profile in simulated
SPI-based nanoparticles were prepared with soy protein-dextran conjugates obtained by
titanium dioxide (TiO2) photocatalysis using a simple ionic gelation method. Formation of the conjugates
was monitored spectrophotometrically for free amino group content (A340nm) and by Fourier transform
infrared spectroscopy (FT-IR). The particles were characterized by for particle size and morphology,
zeta potential, and in vitro release.
Successful glycoconjugation was evidenced by changes in ultraviolet (UV) absorption (A294),
browning, free amino group and FT-IR spectra. The particle size and zeta potential of SPI-dextran
nanoparticles tended to increase and decrease, respectively, with increasing SPI/dextran mass ratio.
Maximum encapsulation and loading efficiencies were around 91.6 and 3.08 %, respectively, which
were significantly higher than for SPI nanoparticles. Successful encapsulation of theophylline in the
polymeric matrix was confirmed by FT-IR spectra. In addition, SPI-dextran nanoparticles reduced
release (p < 0.05) of theophylline in simulated gastric fluid but and enhanced release (p < 0.05) under
simulated intestinal condition.
These results suggest that SPI-dextran nanoparticle formulation is an attractive approach
to achieve encapsulation and controlled release of bioactive substances.
Soy protein; Dextran; Nanoparticles; Encapsulation; Controlled release; Theophylline
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