Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 14, No. 8, 2015, pp. 1393-1398
Bioline Code: pr15182
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 14, No. 8, 2015, pp. 1393-1398
© Copyright 2015 - Tropical Journal of Pharmaceutical Research
Effect of Three Calmodulin Antagonists on Subpopulations of CD44/CD24 Immunophenotypes in Breast Cancer Cell Lines|
Wali, Abdulwahab Noor; El Shal, Mohammed Farouk; Alkhatib, Mayson H. & Damiati, Laila A.
To determine the effect of three calmodulin antagonists (A-7, W-7 and W-13) on the
subpopulations of CD44/CD24 immunophenotypes in MDA-MB-231 and MDA-MB-468 breast cancer
Flow cytometry analysis was used to determine the proportion of the various subpopulations
of the immunophenotypes, viz, CD44+CD24−, CD44−CD24+ and CD44+CD24+, when MDA-MB-231
and MDA-MB-468 cells were subjected to calmodulin antagonists. The effect of W-13 on the invasion
properties of MDA-MB-231 and MDA-MB-468 was investigated using Matrigel invasion assay.
A-7, W-7 and W-13 caused alterations in the subpopulation of CD44+CD24− in MDA-MB-231
cells. The most potent antagonist was W-13 as it reduced the proportion of tumorigenic CD44+CD24− to
0.64 ± 0.05 at a concentration of 80 μM. In contrast, the subpopulation of MDA-MB-468 cells, which had
a low fraction of CD44+CD24−, was not altered when administered with W-7 but showed variations
when incubated with W-13. Specifically, when the concentration of W-13 increased from 20 – 100 μM,
the proportion of CD44+CD24+ was reduced from 92.93 ± 3.2 to 60.96 ± 2.4. The effect of W-13 on the
subpopulations of CD44+CD24− and CD44+CD24+ in MDA-MB-231 and MDA-MB-468, respectively,
reduced the invasion properties of the cells.
The calmodulin antagonist, W-13, has a significant antitumor effect on MDA-MB-231 and
MDA-MB-468 breast cancer cells.
Calmodulin antagonists; Flow cytometry; Invasion assay; Immunophenotypes
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