Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 14, No. 9, 2015, pp. 1605-1611
Bioline Code: pr15209
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 14, No. 9, 2015, pp. 1605-1611
© Copyright 2015 - Tropical Journal of Pharmaceutical Research
Characterization of Polyhydroxyalkanoates Produced by Contaminated Soil Bacteria using Wastewater and Glucose as Carbon Sources|
Munir, Sajida & Jamil, Nazia
Purpose: To isolate polyhydroxyalkanoates (PHA)-producing bacterial strains from contaminated soil
using industrial wastewater and glucose as carbon sources.
Methods: The strains were isolated and identified as Pseudomonas, Bacillus, Enterobacter,
Exiguobacterium and Stenotrophomonas using biochemical tests and further confirmed by Macrogen
sequencing. Two different sources, namely, glucose and wastewater were used to evaluate and
compare the use of wastewater as a carbon source for PHA production. The biomass obtained was
analyzed by Fourier transform infra-red (FTIR) to identify the presence of PHA in it. Afterwards, PHA
extraction was carried out and then gas chromatography (GC) performed to identify PHA monomers.
Results: Utilization of glucose resulted in the production of PHB, while wastewater yielded copolymers
poly-3 hydroxybutyrate-co-3hydroxyvalerate P(3HB-co-3HV) due to its content of volatile fatty acids
such as acetic acid, propionic acid and butyric acid, which led to the production of different types of
polymers. The maximum PHA production was 41 ± 0.22 % obtained for Stenotrophomonas (SM03)
using 2 % glucose as carbon source while for wastewater, maximum production was achieved by the
Pseudomonas strain (SM01).
Conclusion: Wastewater is produced in large quantities daily during various activities and therefore can
be used as a cheap carbon source for the production of valuable products such as PHA.
Polyhydroxyalkanoates; Wastewater; Glucose; Pseudomonas strain; Stenotrophomonas
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