Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 14, No. 9, 2015, pp. 1629-1634
Bioline Code: pr15212
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 14, No. 9, 2015, pp. 1629-1634
© Copyright 2015 - Tropical Journal of Pharmaceutical Research
Effect of Erythropoietin on Microvascular Anastomosis in Rat|
Şentürk, Sadık; Karaçor, Zeynep; Tosun, Zekeriya; Özkan, Adem; Avunduk, M. Cihat & Zümrüt, Murat
Purpose: To investigate the re-endothelialization potential of erythropoietin (EPO) following
microvascular anastomosis in rat femoral artery.
Methods: Ninety-six male Sprague-Dawley rats weighing between 300 g and 320 g were allocated
randomly into two groups (control and EPO, n = 48). Left femoral artery microvascular anastomosis was
performed in the rats. The control group was given 0.5 mL of 0.09 % NaCl subcutaneously 48 h postoperatively
while the EPO group was given 150 U/kg recombinant human erythropoietin subcutaneously
48 h post-operatively. Tissue was harvested from each group after 1, 3, 5, and 7 days. All of the rats
were sacrificed for morphometric analysis. Each arterial segment was analyzed morphometrically using
Clemex Image Analysis software by a single examiner. The cross-sectional area of the media and
neointima was measured and the intima/media ratio was calculated. Hematocrit measurement was
carried out for all the rats.
Results: The I/M ratio differed significantly (p < 0.05) between the control and erythropoietin groups, at
all time-points. The hematocrit was significantly (p < 0.05) lower in the control group than in the
erythropoietin groups on day 7, but not on the other days.
Conclusion: This study demonstrates that erythropoietin markedly inhibits neointima formation with
accelerated re-endothelialization in rat femoral arteries following microvascular anastomosis.
Erythropoietin; Microvascular anastomosis; Re-endothelialization; Hematocrit; Neointima/media ratio
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