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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 14, No. 9, 2015, pp. 1667-1672
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Bioline Code: pr15217
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 14, No. 9, 2015, pp. 1667-1672
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Charge-Transfer Reaction of Cediranib with 2,3-Dichloro-3,5-dicyano-1,4-benzoquinone: Spectrophotometric Investigation and Use in Development of Microwell Assay for Cediranib
Khalil, Nasr Y.; Wani, Tanveer A.; Darwish, Ibrahim A. & Assiri, Ibrahim S.
Abstract
Purpose: To investigate the charge-transfer (CT) reaction between cediranib (CRB) and 2, 3 - dichloro-
5, 6- dicyano -1, 4 - benzoquinone (DDQ) and employment of the reaction as a basis for the
development of a novel 96 - microwell spectrophotometric assay for CRB.
Method: The reaction of CRB and DDQ was investigated in different solvents of varying dielectric
constant and polarity index The reaction resulted in the formation of a red-colored product.
Spectrophotometric investigations confirmed that the reaction proceeded through to the formation of a
(CT) complex.
Results: The molar absorptivity of the CT complex was linearly correlated with the dielectric constant
and polarity index of the solvent; the correlation coefficients were 0.801 and 0.858, respectively. The
association constant of the complex was 0.5 × 103 L mol−1 in 2-propanol. The reaction conditions were
optimized for the development of the microwell assay for CRB. The assay limits of detection and
quantitation were 6.8 and 20.6 μg/well, respectively. The assay was validated as per the guidelines of
the International Conference on Harmonization (ICH) and successfully applied to the analysis of CRB in
its bulk and dosage forms with good accuracy and precision.
Conclusion: The developed assay has high throughput and consumed minimum volume of organic
solvent; thus, it reduces the exposure of the analysts to the toxic effects of organic solvents, and should
significantly lower the analysis cost.
Keywords
Cediranib; 2,3-dichloro-3,5-dicyano-1,4-benzoquinone; Charge-transfer reaction; Spectrophotometry; Microwell assay; High throughput analysis
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