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Dimedone Derivative {2-[(4-Hydroxy-phenylamino)- methylene]-5,5-dimethyl-cyclohexane-1,3-dione} Plays an Important Role in Breast Cancer Treatment
Song, Ling-Qin; Kang, Hua-Feng; Liu, Di; Dai, Zhi-Jun; He, Jian-Jun; Wang, Bao-Feng; Zhao, Yang; Wang, Xi-Jing & Xi, Wen-Tao
Abstract
Purpose: To investigate the effect of 2-[(4-hydroxy-phenylamino)-methylene]-5,5-dimethyl-cyclohex
(HPDH) on mammary carcinogenesis induced by 7,12-dimethylbenz(a)anthracene in female Sprague
Dawley rats.
Methods: Among three groups of rats (50 each) used in the study, the control group was fed standard
diet alone, ibuprofen group received standard diet containing 1200 mg/kg ibuprofen while HPDH group
was administered standard diet containing 1200 mg/kg HPDH. The treatment was for 10 days for all the
groups. All the animals received 20 mg of DMBA intragastrically by gavage. Clinical parameters for the
rats were recorded weekly. Micrometer caliper was used to measure the diameter of all the tumors at
the end of the experiment and tumor volume calculated. Histological evaluation was performed using
hematoxylin and eosin (H&E) staining. High performance liquid chromatography (HPLC) was used to
determine the level of HPDH and ibuprofen in the serum of the animals.
Results: The data revealed a significant decrease in the number of rats with mammary tumor, number
of tumors/rat and tumor volume by 54, 72 and 75 %, respectively, in HPDH group compared to control
group. The ibuprofen-treated rats also showed significant decrease in the number of rats with tumor,
number of tumors/rat and tumor volume by 43, 55, and 59 %, respectively. Treatment of rats with HPDH
increased the latency period of tumor induction significantly (p < 0.005). Median detection period (50 %
of tumors) was 92, 83 and 56 days, respectively, in HPDH, ibuprofen and control groups, respectively,
after DMBA induction.
Conclusion: These results demonstrate that HPDH possesses strong chemopreventive activity against
mammary carcinogenesis.
Keywords
Carcinogenesis; Mammary tumor; Median detection period; Tumor; Latency period; Chemopreventive activity; Ibuprofen
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