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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996 EISSN: 1596-5996
Vol. 14, No. 10, 2015, pp. 1779-1785
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Bioline Code: pr15232
Full paper language: English
Document type: Research Article
Document available free of charge
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Tropical Journal of Pharmaceutical Research, Vol. 14, No. 10, 2015, pp. 1779-1785
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Apoptotic Potential of Artemsia sieberia Besser (Asteraceae) Fraction against Human Cancer Cell Lines
Abutaha, Nael; Mashaly, Ashraf M. A.; Farooq, Muhammad & Wadaan, Muhammad A.
Abstract
Purpose: To investigate the anti-proliferative and apoptotic activity of crude and dichloromethane
fraction of Artemsia sieberia against seven cancer cell lines (Colo20, HCT116, DLD, MCF7, Jurkat, HepG2 and
L929).
Methods: A. sieberi was extracted with methanol and further purification was carried out using liquidliquid
extraction with hexane, dichloromethane and ethyl acetate. Each extract was assayed for
cytotoxic potential against cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
Bromide (MTT) assay. The morphology of the HepG2 cell nucleus was investigated by Hoechst 33342,
DNA-binding dye. A Tali™ image-based cytometer was used to assess cell viability, death and
apoptosis using annexin-v /pi (propidium iodide). A chromatographic fingerprint was constructed using
high performance liquid chromatography (HPLC).
Results: The most effective anticancer activity of the unrefined methanol extract was against HepG2
cell lines (LC50 = 161.5 μg/mL). The hexane and ethyl acetate fractions showed no antiproliferative
activity. The dichloromethane fraction displayed higher cytotoxic activity (LC50 = 61.75 μg/mL) and also
repressed the migration of the cells. About 50 % of HepG2 cells were apoptotic when treated for 24 h
with the dichloromethane fraction at the concentration of 120 μg/mL
Conclusion: A. sieberi possesses apoptotic activity and inhibited the migration of the HepG2 cell lines.
Keywords
Artemsia Sieberia; Apoptosiss; Cytotoxicity; Hoescht staining; HepG2 cell lines
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