To determine the phenolic composition and antiproliferative activity of 16 different extracts
(hexane, dichloromethane, methanol and water) obtained from
on human breast cancer (MCF-7) and human
hepatocellular carcinoma (HepG2/C3A) cell lines.
The aerial parts of the plants were successively extracted with hexane, dichloromethane,
methanol and water using a
Soxhlet apparatus. The phenolic content of the plants were determined by
plants by high performance liquid chromatography (HPLC) while their antiproliferative activity was
evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, a yellow tetrazole (MTT)
Among the tested extracts, the methanol extract of B. perennis
showed the best antiproliferative
activity against MCF-7 cell line with IC50
(inhibiting 50 % of cell growth) value of 71.6 μg/mL.
Furthermore, the dichloromethane extract of C. galaticus
showed the best anti-proliferative activity
against HepG2/C3A cell line with IC50
of 57.3 μg/mL. The HPLC data for the plant extracts showed the
presence of the following phenolic compounds: gallic acid monohydrate, caffeic acid, rutin hydrate,
luteolin-7-O-β-D glucoside, kaempferol, myricetin, quercetin, coumarin and apigenin.
The findings of this study indicate that there is some justification for the use of B. perennis
and C. galaticus
as traditional anticancer medicinal herbs.