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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 10, 2015, pp. 1787-1795
Bioline Code: pr15233
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 10, 2015, pp. 1787-1795

 en Antiproliferative Activity of Some Medicinal Plants on Human Breast and Hepatocellular Carcinoma Cell Lines and their Phenolic Contents
Karakas, Fatma Pehlivan; Yildirim, Arzu Birinci; Bayram, Recep; Yavuz, Muhsine Z.; Gepdiremen, Akcahan & Turker, Arzu Ucar

Abstract

Purpose: To determine the phenolic composition and antiproliferative activity of 16 different extracts (hexane, dichloromethane, methanol and water) obtained from Bellis perennis check for this species in other resources , Convolvulus galaticus check for this species in other resources , Trifolium pannonicum check for this species in other resources and Lysimachia vulgaris check for this species in other resources on human breast cancer (MCF-7) and human hepatocellular carcinoma (HepG2/C3A) cell lines.
Methods: The aerial parts of the plants were successively extracted with hexane, dichloromethane, methanol and water using a Soxhlet apparatus. The phenolic content of the plants were determined by plants by high performance liquid chromatography (HPLC) while their antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, a yellow tetrazole (MTT) assay.
Results: Among the tested extracts, the methanol extract of B. perennis showed the best antiproliferative activity against MCF-7 cell line with IC50 (inhibiting 50 % of cell growth) value of 71.6 μg/mL. Furthermore, the dichloromethane extract of C. galaticus showed the best anti-proliferative activity against HepG2/C3A cell line with IC50 of 57.3 μg/mL. The HPLC data for the plant extracts showed the presence of the following phenolic compounds: gallic acid monohydrate, caffeic acid, rutin hydrate, luteolin-7-O-β-D glucoside, kaempferol, myricetin, quercetin, coumarin and apigenin.
Conclusion: The findings of this study indicate that there is some justification for the use of B. perennis and C. galaticus as traditional anticancer medicinal herbs.

Keywords
Bellis perennis; Convolvulus galaticus; Trifolium pannonicum subsp. elongatum; Lysimachia vulgaris; MCF-7; HepG2/C3A; Phenolics; Breast cancer; Antiproliferative

 
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