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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 10, 2015, pp. 1803-1811
Bioline Code: pr15235
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 10, 2015, pp. 1803-1811

 en Effect of 3,3′-Biisofraxidin on Apoptosis of Human Gastric Cancer BGC-823 Cells
Wu, Jian-Tao; Lv, She-Min; Lu, Chun-Hui; Gong, Jun & An, Jian-Bo


Purpose: To study the effect of 3,3′-biisofraxidin from Sarcandrae Herba check for this species in other resources on the proliferation of BGC- 823 cells and the possible mechanisms.
Methods: Cell Counting Kit-8 (CCK-8), flow cytometry, Western blot and xenograft assays were used to determine the effects of 3,3′-biisofraxidin on the proliferation, apoptosis, apoptotic proteins and xenograft of BGC-823 cells.
Results: 3,3′-Biisofraxidin significantly (p < 0.01) inhibited the proliferation of BGC-823 cells (concentrations: 10 - 40 μM; cell viability: 30.45 - 76.68 % in CCK-8 assay) with half maximal inhibitory concentration (IC50) value of 20.35 μM and induced the apoptosis of BGC-823 cells (concentrations: 10, 20 and 40 μM; apoptotic cells: 11.92, 20.10 and 33.64 % in flow cytometry assay), compared with the control (cell viability: 99.73 %; apoptotic cells: 5.18 %). 3,3′-Biisofraxidin (10, 20 and 40 μM in vitro; 40 mg/kg in vivo) significantly (p < 0.05 or 0.01) down-regulated the expressions of anti-apoptotic proteins (Bcl-2, Bcl-xl and Survivin) and up-regulated the expressions of pro-apoptotic proteins (Smac, caspase-3, caspase-7 and caspase-9), compared with the control. Moreover, the release of cytochrome c from the mitochondria to the cytoplasm was significantly (p < 0.01) promoted in vitro, compared with the control. 3,3′-Biisofraxidin (40 mg/kg) significantly (p < 0.05 or 0.01) inhibited the growth of tumor in xenograft assay, compared with the control.
Conclusion: 3,3′-Biisofraxidin significantly induces the apoptosis of BGC-823 cells in vitro and in vivo through the mitochondria-mediated apoptotic pathway, and therefore has a potential to be developed into an anti-gastric cancer drug.

Sarcandrae Herba; Gastric cancer; 3,3′-Biisofraxidin; Mitochondria-mediated apoptotsis; Cell Counting Kit-8; Xenograft

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