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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 10, 2015, pp. 1813-1820
Bioline Code: pr15236
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 10, 2015, pp. 1813-1820

 en Anticancer Effects of Chenopodium ambrosiodes check for this species in other resources L. Essential Oil on Human Breast Cancer MCF-7 Cells In vitro
Ya-Nan, Wang; Jia-Liang, Wu; Dan-Wei, Ma; Jiao, Li & Du-Yu, Du-Yu

Abstract

Purpose: To investigate the most effective compound of C. ambrosiodes check for this species in other resources essential oil for the induction of cell death in human breast cancer cells (MCF-7), and the mechanism of induction.
Methods: MCF-7 cells were treated with essential oil and its two main components, 1-isopropyl-4- methylbenzene and α-terpinene, respectively, for 24 and 48 h in vitro. To determine their cytotoxicity on MCF-7 cells, in vitro cytotoxicity, 3-(4, 5-dimethylthaizol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and live/dead cell fluorescent staining were used. MCF-7 cellular superoxide dismutase (SOD), catalase (CAT) vitality and malondialdehyde (MDA) content were also evaluated.
Results: MTT results showed that essential oil and its two main compositions significantly inhibited the growth of MCF-7 cells in 24 h (p < 0.05), which was consistent with the Live/dead cell fluorescent staining results. After 24 h incubation the average inhibition rate is 58.98 % for essential oil, 37.8 % for 1-isopropyl-4-methylbenzene and 32.09 % for α-terpinene. With increase in the concentration of essential oil and the two main components, the relative activity of SOD significantly decreased (p < 0.05), while the relative activity of CAT was gradually increased (p < 0.05), compared with control. MDA relative content significantly increased (p < 0.05) until the concentration was 1.25, 0.21 and 0.17 μg/ml for essential oil , 1-isopropyl-4-methylbenzene and α-terpinene , and thereafter significantly decreased (p < 0.05) , compared to control.
Conclusion: The data suggest that the essential oil of C. ambrosioides and its two main components inhibit MCF-7 cell proliferation cell death by inducing oxidative damage. However, the two main components are less effective in their anticancer activity than the essential oil

Keywords
Chenopodium ambrosioides L. Essential oil; 1-isopropyl-4-methylbenzene; α-Terpinene; Breast cancer MCF-7 cells; Antitumor activity

 
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