To investigate the toxicological profile of the ethanol extract of
acute and sub-chronic administration to rodents.
In the acute toxicity study, a single administration of the extract at doses of 2000 and 5000
mg/kg, respectively, was given to the mice. Mice were observed for general behavioural changes,
adverse effects and mortality up to 14 days post-treatment. In sub-acute toxicity studies, EEOS was
given orally to male and female rats at doses of 250 mg/kg, 500 mg/kg and 1000 mg/kg for 28 days.
Body weight, food intake and water intake were monitored throughout the experimental period;
hematological parameters, biochemical parameters of the blood and histopathology of various organs
were also evaluated at the end of the experiment.
In the acute toxicity study, both the doses used did not cause any mortality or significant
behavioural changes. Daily oral administration of EEOS for 28 days, in the sub-chronic toxicity study,
did not show any treatment-related abnormalities with regard to hematological and biochemical
parameters. There were no significant differences in body weight and organ weight between the control
and treated groups (p < 0.05). Histological analysis did not show any morphological changes in the
major vital organs (liver, kidney, stomach, spleen, brain and heart) tested.
These results demonstrate that the extract may not have any single dose toxicity. The
LD50 value is greater than 5000 mg/kg. The no-observed adverse effects level (NOAEL) was
considered to be 1000 mg/kg for male and female rats, respectively.