Purpose: To determine the effect alcohol extract of
Zataria multiflora,
Satureja bachtiarica
and
Zaravschanica membranacea on immunohematologic factors in Wistar rats.
Methods: Wistar rats were randomly allocated to seven treatment groups which consisted of control
group with water and feed only (1); 200 mg. kg
-1 Z. membranacea (2); 400 mg. kg
-1 Z. membranacea
(3); 200 mg. kg
-1 S. bachtiarica (4); 400 mg. kg
-1 S. bachtiarica (5); 200 mg. kg
-1 Z. multiflora (6) and
400 mg. kg
-1 Z. multiflora (7). Erythrocyte counts (RBC), packed cell volumes (PCV), haemoglobin (Hb)
concentration, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean
corpuscular hemoglobin concentration (MCHC), Translocation and Assembly Module (Tam) protein, IgM
and albumin were measured after 29 days.
Results: Z. membranacea at 200 mg kg
-1 showed the highest level of Tam-protein content (p < 0.05).
Z.
multiflora boiss at 400 mg kg
-1 produced higher levels of immunoglobulin M (IgM) compared to
S.
bachtiarica and
Z. multiflora (p < 0.05). Both
Z. membranacea and
S. bachtiarica at 200 mg. kg
-1 caused
a significant increase in albumin levels in the rats (p < 0.05).
Z. multiflora at 400 mg. kg
-1 had the
highest effect on white blood cells (WBC) while
S. bachtiarica produced the highest effect on neutrophils
(Nut) (p < 0.05).
Z. membranacea and
Z. multiflora at 200 mg. kg
-1 showed significantly higher levels of
monocytes (Mon) % (p < 0.05).
Z. multiflora and
S. bachtiarica at 400 mg. kg
-1 showed a significant
effect on phagocytosis % (p < 0.05) whilst
S. bachtiarica at 400 mg.kg
-1 had a significant effect on
phagocytosis number (p < 0.05).
Conclusion: The alcohol extracts of
Z. multiflora Z. membranacea and
S. bachtiarica extracts are
capable of stimulating the immune defense mechanism without causing undesirable effects on
hematological parameters.
