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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 11, 2015, pp. 2091-2097
Bioline Code: pr15274
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 11, 2015, pp. 2091-2097

 en Effect of the Extract of Endophytic fungus, Nigrospora sphaerica check for this species in other resources CL-OP 30, Against the Growth of Methicillin- Resistant Staphylococcus aureus check for this species in other resources (MRSA) and Klebsiella pneumonia check for this species in other resources cells
Ibrahim, Darah; Lee, Chong Chai; Yenn, Tong Woei; Zakaria, Latiffah & Sheh-Hong, Lim

Abstract

Purpose: To investigate the effect of the ethyl acetate extract of endophytic fungus, Nigrospora sphaerica check for this species in other resources CL-OP 30 against the growth of methicillin-resistant Staphylococcus aureus check for this species in other resources (MRSA) and Klebsiella pneumonia check for this species in other resources cells.
Methods: Time-kill assay was used to examine the effect of the extract on the bacterial growth profile. The effects of extract on ultrastructure of MRSA and K. pneumoniae cells were analyzed by scanning electron microscope (SEM) and transmission electron microscope (TEM).
Results: The time-kill test revealed that the bacteriocidal activity of the extract was both concentrationand time-dependent. After 12 h treatment, interaction of extract with MRSA cells resulted in the formation of pit, disintegration of cell wall and membrane, and ultimately cell death, while K. pneumoniae cells became crumpled, and the cell walls and membranes disintegrated, resulting in leakage of their cytoplasmic contents.
Conclusion: These data suggest that the Nigrospora sphaerica CL-OP 30 extract principally affects the cell wall in growing MRSA and K. pneumonia cells.

Keywords
Endophytic fungus; Nigrospora sphaerica; Antimicrobial activity; Cellular structure degeneration

 
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