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Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
ISSN: 1596-5996
EISSN: 1596-9827
Vol. 14, No. 11, 2015, pp. 2099-2106
Bioline Code: pr15275
Full paper language: English
Document type: Research Article
Document available free of charge

Tropical Journal of Pharmaceutical Research, Vol. 14, No. 11, 2015, pp. 2099-2106

 en Antibacterial Activity and Mode of Action of Mentha arvensis check for this species in other resources Ethanol Extract against Multidrug-Resistant Acinetobacter baumannii check for this species in other resources
Zhang, Ling; Xu, Shu-gen; Liang, Wei; Mei, Jun; Di, Yue-ying; Lan, Hui-hua; Yang, Yan; Wang, Wei-wei; Luo, Yuan-yuan & Wang, Hou-zhao

Abstract

Purpose: To evaluate the antibacterial effect of ethanol extract of Mentha arvensis check for this species in other resources against multi-drug resistant Acinetobacter baumannii check for this species in other resources using liquid chromatography–mass spectrometry (LC-ESI-MS).
Methods: Disc diffusion and microdilution assays were used to evaluate the antibacterial effect of the extract by measuring the zone of inhibition, minimum inhibitory concentration (MIC) and and minimum bacteriocidal concentration (MBC) of the extract against the test bacteria. Scanning electron microscopy (SEM) was employed to evaluate the morphological changes induced by the extract in cellular membrane of the bacteria. Reactive oxygen species (ROS) generation and protein leakage from the bacterial cells induced by the extract were also evaluated.
Results: The extract showed dose-dependent growth inhibitory effects against A. baumannii with MIC and MBC of 23.5 and 72.1 μg/mL, respectively. The extract also induced potent ROS generation and protein leakage in A. baumannii bacterial cells. SEM findings revealed that the extract induced potential cellular damage which increased with increasing extract concentration.
Conclusion: The ethanol extract of Mentha arvensis is a potent antibacterial agent against A. baumannii and acts by inducing lethal cellular damage to the bacterium.

Keywords
Mentha arvensis; Acinetobacter baumannii; Reactive oxygen species; Antibacterial activity; Cellular membrane damage

 
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