Tropical Journal of Pharmaceutical Research
Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, Nigeria
Vol. 14, No. 12, 2015, pp. 2231-2237
Bioline Code: pr15294
Full paper language: English
Document type: Research Article
Document available free of charge
Tropical Journal of Pharmaceutical Research, Vol. 14, No. 12, 2015, pp. 2231-2237
© Copyright 2015 - Tropical Journal of Pharmaceutical Research
Extraction-condition Optimization of Baicalein and Schisandrin from Hu-gan-kang-yuan Formula Using Orthogonal Array Design|
Wei, Feng Huan; Chen, Fei Long; Shen, Qun & Chen, MinTing
Purpose: To optimize the extraction conditions for Hu-gan-kang-yuan Formula based on extraction
rates of baicalein and schisandrin using an orthogonal array design.
Methods: Ethanol concentration (50 - 70 %), ratio of solvent to raw material (8 - 12 mL/g), and
extraction time (1 - 3 h) were examined with a three-factor and three-level L9(3)3 orthogonal array
design. In addition, analysis of variance (ANOVA) was used to evaluate the statistical significance of the
effects of individual factors on extraction rates of baicalein and schisandrin determined by high
performance liquid chromatography (HPLC). The number of extractions was further investigated to
confirm the extraction rate of target compounds based on the optimized conditions.
Results: The optimized conditions were an ethanol concentration of 70 %; ratio of solvent to raw
material, 12:1 mL/g; and extraction time of 60 min. Ethanol concentration and ratio of solvent to raw
material showed significant effects on the extraction of the two compounds (p < 0.05). The number of
extraction steps, two, was reasonable. The final optimum extraction conditions resulted in 79.48 ± 1.40
and 88.55 ± 1.85 % of extraction for baicalein and schisandrin, respectively.
Conclusion: The optimized extraction conditions for baicalein and schisandrin are practicable and
repeatable, and can be upgraded for pilot-scale production of Hu-gan-kang-yuan preparations.
Hu-gan-kang-yuan Formula; Extract-condition optimization; Orthogonal array design; Baicalein; Schisandrin
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